Abstract
We have cloned six different class I genes from a B10.P sperm library. After cotransfection with the herpes simplex tk gene, one L-cell line was found to react with six H-2Dp-specific monoclonal antibodies. The cell line L12a did not react with Kp-specific monoclonal antibodies. This identification was confirmed by mapping a 2.5 kb Bam H 1 restriction fragment present in the λ12a DNA clone to the D-TL region of H-2p. Only a single 8.8 kb Barn H1 fragment can be assigned to Kp by restriction fragment length polymorphism, while many others map to the D-TL interval. A restriction map of λ12a is presented.
Original language | English (US) |
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Pages (from-to) | 195-204 |
Number of pages | 10 |
Journal | Immunogenetics |
Volume | 19 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1984 |
ASJC Scopus subject areas
- Immunology
- Genetics