Cloning and expression of a human kidney cDNA for an α2-adrenergic receptor subtype

J. W. Regan; T. S. Kobilka; T. L. Yang-Feng; M. G. Caron; R. J. Lefkowitz; B. K. Kobilka

doi:10.1073/pnas.85.17.6301

Cloning and expression of a human kidney cDNA for an α₂-adrenergic receptor subtype

J. W. Regan, T. S. Kobilka, T. L. Yang-Feng, M. G. Caron, R. J. Lefkowitz, B. K. Kobilka

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Abstract

An α₂-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet α₂-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet α₂-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the α₂-adrenergic ligand [³H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the α₂B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet α₂-adrenergic receptor (α₂A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective α-adrenergic ligands.

Original language	English (US)
Pages (from-to)	6301-6305
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	17
DOIs	https://doi.org/10.1073/pnas.85.17.6301
State	Published - 1988
Externally published	Yes

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title = "Cloning and expression of a human kidney cDNA for an α2-adrenergic receptor subtype",

abstract = "An α2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet α2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet α2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the α2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the α2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet α2-adrenergic receptor (α2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective α-adrenergic ligands.",

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year = "1988",

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AU - Regan, J. W.

AU - Kobilka, T. S.

AU - Yang-Feng, T. L.

AU - Caron, M. G.

AU - Lefkowitz, R. J.

AU - Kobilka, B. K.

PY - 1988

Y1 - 1988

N2 - An α2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet α2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet α2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the α2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the α2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet α2-adrenergic receptor (α2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective α-adrenergic ligands.

AB - An α2-adrenergic receptor subtype has been cloned from a human kidney cDNA library using the gene for the human platelet α2-adrenergic receptor as a probe. The deduced amino acid sequence resembles the human platelet α2-adrenergic receptor and is consistent with the structure of other members of the family of guanine nucleotide-binding protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7), and the α2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis with a variety of adrenergic ligands suggests that this cDNA clone represents the α2B-adrenergic receptor. The gene for this receptor is on human chromosome 4, whereas the gene for the human platelet α2-adrenergic receptor (α2A) lies on chromosome 10. This ability to express the receptor in mammalian cells, free of other adrenergic receptor subtypes, should help in developing more selective α-adrenergic ligands.

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Cloning and expression of a human kidney cDNA for an α₂-adrenergic receptor subtype

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