Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice

Liana Asatryan; Sheraz Khoja; Kathleen E. Rodgers; Ronald L. Alkana; Hidekazu Tsukamoto; Daryl L. Davies

doi:10.1016/j.jneuroim.2015.06.007

Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice

Liana Asatryan, Sheraz Khoja, Kathleen E. Rodgers, Ronald L. Alkana, Hidekazu Tsukamoto, Daryl L. Davies

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.

Original language	English (US)
Pages (from-to)	169-179
Number of pages	11
Journal	Journal of Neuroimmunology
Volume	285
DOIs	https://doi.org/10.1016/j.jneuroim.2015.06.007
State	Published - 2015
Externally published	Yes

Keywords

Astrocytes
High fat diet
Intragastric ethanol feeding
Microglia
Neuroinflammation and neuronal loss
Pro-inflammatory cytokines
Purinergic P2X7 receptors

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2015.06.007

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@article{41bdf4a6b7ca43ccac624b87501bf369,

title = "Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice",

abstract = "The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.",

keywords = "Astrocytes, High fat diet, Intragastric ethanol feeding, Microglia, Neuroinflammation and neuronal loss, Pro-inflammatory cytokines, Purinergic P2X7 receptors",

author = "Liana Asatryan and Sheraz Khoja and Rodgers, \{Kathleen E.\} and Alkana, \{Ronald L.\} and Hidekazu Tsukamoto and Davies, \{Daryl L.\}",

note = "Funding Information: Sources of support: Zumberge Individual Research Fund (USC) and NIH/NIAAA AA017243 (to L.A.), NIH/NIAAA A022448 (to D.L.D.), NIH HL08722 (to K.R.), NIH P50AA011999 and VA 1I01BX001991 (to H.T.). Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

doi = "10.1016/j.jneuroim.2015.06.007",

language = "English (US)",

volume = "285",

pages = "169--179",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

}

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T1 - Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice

AU - Asatryan, Liana

AU - Khoja, Sheraz

AU - Rodgers, Kathleen E.

AU - Alkana, Ronald L.

AU - Tsukamoto, Hidekazu

AU - Davies, Daryl L.

N1 - Funding Information: Sources of support: Zumberge Individual Research Fund (USC) and NIH/NIAAA AA017243 (to L.A.), NIH/NIAAA A022448 (to D.L.D.), NIH HL08722 (to K.R.), NIH P50AA011999 and VA 1I01BX001991 (to H.T.). Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015

Y1 - 2015

N2 - The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.

AB - The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.

KW - Astrocytes

KW - High fat diet

KW - Intragastric ethanol feeding

KW - Microglia

KW - Neuroinflammation and neuronal loss

KW - Pro-inflammatory cytokines

KW - Purinergic P2X7 receptors

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DO - 10.1016/j.jneuroim.2015.06.007

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AN - SCOPUS:84943275410

SN - 0165-5728

VL - 285

SP - 169

EP - 179

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

ER -

Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice

Abstract

Keywords

ASJC Scopus subject areas

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