Chronic ethanol exposure combined with high fat diet up-regulates P2X7 receptors that parallels neuroinflammation and neuronal loss in C57BL/6J mice

Liana Asatryan, Sheraz Khoja, Kathleen E. Rodgers, Ronald L. Alkana, Hidekazu Tsukamoto, Daryl L. Davies

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The present investigation tested the role of ATP-activated P2X7 receptors (P2X7Rs) in alcohol-induced brain damage using a model that combines intragastric (iG) ethanol feeding and high fat diet in C57BL/6J mice (Hybrid). The Hybrid paradigm caused increased levels of pro-inflammatory markers, changes in microglia and astrocytes, reduced levels of neuronal marker NeuN and increased P2X7R expression in ethanol-sensitive brain regions. Observed changes in P2X7R and NeuN expression were more pronounced in Hybrid paradigm with inclusion of additional weekly binges. In addition, high fat diet during Hybrid exposure aggravated the increase in P2X7R expression and activation of glial cells.

Original languageEnglish (US)
Pages (from-to)169-179
Number of pages11
JournalJournal of Neuroimmunology
Volume285
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • Astrocytes
  • High fat diet
  • Intragastric ethanol feeding
  • Microglia
  • Neuroinflammation and neuronal loss
  • Pro-inflammatory cytokines
  • Purinergic P2X7 receptors

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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