TY - JOUR
T1 - Chromatographic characterization of vasoactive intestinal polypeptide in Guinea pig and rhesus monkey eyes
AU - Stone, Richard A.
AU - Wilson, Cheryl M.
AU - Glembotski, Christopher C.
N1 - Funding Information:
Multiple immunoreactive forms of VIP have been found in the gut (14). Because immunohistochemical reports in the eye differ regarding the extent of ocular VIP nerve fibers, we used for radioimmunoassay an antiserum that stains a broad distribution of intraocular nerve fibers when used for histochemistry (15). In each of the ocular tissues studied, a single major peak of VIP immunoreactivity was detected, similar to the porcine VIP standard. Only in the guinea pig anterior uvea or choroid did we observe a somewhat broadened or additional smaller peak by RP-HPLC. Despite the control experiments, the chromatographic characteristics of immunoreactive VIP in the guinea pig uvea may still result from metabolism during extraction but alternatively may represent another immunoreactive form of the peptide. Regardless of the explanation, we conclude that a single form of the peptide, chromatographically similar to porcine VIP, predominates in the anterior uvea, choroid and retina of the guinea pig and the rhesus monkey ACKNOWLEDGMENTS Dr. James H. Vickers, John Cogan and their associates at the Bureau of Biologics of the Food and Drug Administration provided valuable assistance in obtaining the monkey eyes. Supported by NIH Grants EY-05454, NS-25037 and by the Harry and Edith H. Hubschman Research Fund of the Scheie Eye Institute.
PY - 1990
Y1 - 1990
N2 - Acid extracts of guinea pig and rhesus monkey anterior uvea, choroid and retina contain immunoreactive VIP. By reversed phase high performance liquid chromatography, the immunoreactive material from these ocular tissues elutes at a position similar to synthetic porcine VIP. Only in the guinea pig anterior uvea is a second smaller peak detected. By size exclusion high performance liquid chromatography, the peptide in the monkey anterior uvea, choroid and retina elutes in the identical position as the synthetic porcine VIP standard with an apparent molecular weight of 3450 daltons. We conclude that a single form of VIP, chromatographically similar to the porcine standard, is the predominant form of the peptide in the eye of guinea pig and rhesus monkey.
AB - Acid extracts of guinea pig and rhesus monkey anterior uvea, choroid and retina contain immunoreactive VIP. By reversed phase high performance liquid chromatography, the immunoreactive material from these ocular tissues elutes at a position similar to synthetic porcine VIP. Only in the guinea pig anterior uvea is a second smaller peak detected. By size exclusion high performance liquid chromatography, the peptide in the monkey anterior uvea, choroid and retina elutes in the identical position as the synthetic porcine VIP standard with an apparent molecular weight of 3450 daltons. We conclude that a single form of VIP, chromatographically similar to the porcine standard, is the predominant form of the peptide in the eye of guinea pig and rhesus monkey.
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U2 - 10.3109/02713689009044525
DO - 10.3109/02713689009044525
M3 - Article
C2 - 2347206
AN - SCOPUS:0025360286
SN - 0271-3683
VL - 9
SP - 287
EP - 291
JO - Current Eye Research
JF - Current Eye Research
IS - 3
ER -