TY - CHAP
T1 - Chromatin Immunoprecipitation Assay to Analyze the Effect of G-Quadruplex Interactive Agents on the Binding of RNA Polymerase II and Transcription Factors to a Target Promoter Region
AU - Sun, Daekyu
N1 - Funding Information:
This work was supported by NIH grant CA109069 and American Cancer Society grant RSGM-12-046-01-CDD to D.S.
Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Growing evidence suggests the existence of G-quadruplexes and their involvement in transcriptional regulation of many human genes, including VEGF. These studies also provide strong evidence that G-quadruplex structures are stabilized by binding to small molecules, resulting in the modulation of the transcription of genes whose promoters form G-quadruplexes. Here, we describe a chromatin immunoprecipitation (ChIP) assay to determine whether G-quadruplex-interactive agents influence the recruitment of cellular transcription factors, such as Sp1, nucleolin, or hnRNP-K to target genes that contain potential G-quadruplex (G4)-forming sequences in their promoters, subsequently modulating the occupancy of RNA Pol II on the same promoter region.
AB - Growing evidence suggests the existence of G-quadruplexes and their involvement in transcriptional regulation of many human genes, including VEGF. These studies also provide strong evidence that G-quadruplex structures are stabilized by binding to small molecules, resulting in the modulation of the transcription of genes whose promoters form G-quadruplexes. Here, we describe a chromatin immunoprecipitation (ChIP) assay to determine whether G-quadruplex-interactive agents influence the recruitment of cellular transcription factors, such as Sp1, nucleolin, or hnRNP-K to target genes that contain potential G-quadruplex (G4)-forming sequences in their promoters, subsequently modulating the occupancy of RNA Pol II on the same promoter region.
KW - Chromatin immunoprecipitation assay
KW - G-quadruplex-interactive agents
KW - G-quadruplexes
KW - NSC82892
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=85071426494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071426494&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9666-7_13
DO - 10.1007/978-1-4939-9666-7_13
M3 - Chapter
C2 - 31444753
AN - SCOPUS:85071426494
T3 - Methods in Molecular Biology
SP - 233
EP - 242
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -