Cholesterol in high-density lipoprotein: use of Mg2+/dextran sulfate in its enzymic measurement

P. R. Finley; R. B. Schifman; R. J. Williams; D. A. Lichti

doi:10.1093/clinchem/24.6.931

Cholesterol in high-density lipoprotein: use of Mg²⁺/dextran sulfate in its enzymic measurement

P. R. Finley, R. B. Schifman, R. J. Williams, D. A. Lichti

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380 Scopus citations

Abstract

The authors describe a method for measuring high-density lipoprotein cholesterol. MgCl₂ and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/liter. The precision of the method is excellent in the range of clinical interest (100 to 1000 mg of cholesterol per liter). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of two laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples, and agreement was good.

Original language	English (US)
Pages (from-to)	931-933
Number of pages	3
Journal	Clinical chemistry
Volume	24
Issue number	6
DOIs	https://doi.org/10.1093/clinchem/24.6.931
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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title = "Cholesterol in high-density lipoprotein: use of Mg2+/dextran sulfate in its enzymic measurement",

abstract = "The authors describe a method for measuring high-density lipoprotein cholesterol. MgCl2 and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/liter. The precision of the method is excellent in the range of clinical interest (100 to 1000 mg of cholesterol per liter). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of two laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples, and agreement was good.",

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language = "English (US)",

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journal = "Clinical chemistry",

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T1 - Cholesterol in high-density lipoprotein

T2 - use of Mg2+/dextran sulfate in its enzymic measurement

AU - Finley, P. R.

AU - Schifman, R. B.

AU - Williams, R. J.

AU - Lichti, D. A.

PY - 1978

Y1 - 1978

N2 - The authors describe a method for measuring high-density lipoprotein cholesterol. MgCl2 and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/liter. The precision of the method is excellent in the range of clinical interest (100 to 1000 mg of cholesterol per liter). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of two laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples, and agreement was good.

AB - The authors describe a method for measuring high-density lipoprotein cholesterol. MgCl2 and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the cholesterol concentration of which is estimated by an enzymic method, with a discrete analyzer (Abbott Bichromatic Analyzer). Concentration and instrument response are linearly related to 50 mg/liter. The precision of the method is excellent in the range of clinical interest (100 to 1000 mg of cholesterol per liter). The precision and efficiency of the precipitation are shown at various concentrations of high-density lipoprotein cholesterol. The method was compared to that of two laboratories in the Cooperative Lipoprotein Phenotyping Study group by testing a number of split samples, and agreement was good.

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Cholesterol in high-density lipoprotein: use of Mg²⁺/dextran sulfate in its enzymic measurement

Abstract

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