Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography

Paul B. Myrdal; B. Steven Angersbach; Kelly Karlage; Philip J. Kuehl

doi:10.1016/j.chroma.2006.07.010

Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography

Paul B. Myrdal, B. Steven Angersbach, Kelly Karlage, Philip J. Kuehl

Pharmacy Practice and Science

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.

Original language	English (US)
Pages (from-to)	315-319
Number of pages	5
Journal	Journal of Chromatography A
Volume	1132
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2006.07.010
State	Published - Nov 3 2006

Keywords

Arachidonic acid metabolism
Chiral separation
Chiralcel OR-RH
HETEs
Lipoxygenase

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2006.07.010

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title = "Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography",

abstract = "An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.",

keywords = "Arachidonic acid metabolism, Chiral separation, Chiralcel OR-RH, HETEs, Lipoxygenase",

author = "Myrdal, {Paul B.} and Angersbach, {B. Steven} and Kelly Karlage and Kuehl, {Philip J.}",

note = "Funding Information: The authors would like to graciously thank the Arizona Cancer Center, along with the American Cancer Society, grant number IRG 7400128, for funding this research.",

year = "2006",

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doi = "10.1016/j.chroma.2006.07.010",

language = "English (US)",

volume = "1132",

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T1 - Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography

AU - Myrdal, Paul B.

AU - Angersbach, B. Steven

AU - Karlage, Kelly

AU - Kuehl, Philip J.

N1 - Funding Information: The authors would like to graciously thank the Arizona Cancer Center, along with the American Cancer Society, grant number IRG 7400128, for funding this research.

PY - 2006/11/3

Y1 - 2006/11/3

N2 - An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.

AB - An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.

KW - Arachidonic acid metabolism

KW - Chiral separation

KW - Chiralcel OR-RH

KW - HETEs

KW - Lipoxygenase

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EP - 319

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1-2

ER -

Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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