Chemical Probes to Interrogate the Extreme Environment of Mosquito Larval Guts

Lindsay E. Guzmán, Anjalee N. Wijetunge, Brendan F. Riske, Brooke B. Massani, Michael A. Riehle, John C. Jewett

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Mosquito control methods are vital to curtail the spread of life-threatening illnesses, such as dengue fever, malaria, and yellow fever. Vector control technologies must be selective to minimize deleterious effects on our ecosystem. Successful methods that control mosquito larva populations utilize the uniquely high alkaline nature of the midgut. Here, we present novel protected triazabutadienes (pTBD) that are deprotected under basic conditions of the larval midgut, releasing an aryl diazonium ion (ADI) that results in protein modification. The probes contain a bioorthogonal terminal alkyne handle, enabling a selective Cu-click reaction with an azidofluorophore for quantification by SDS PAGE and visualization using fluorescence microscopy. A control TBD, unable to release an ADI, did not label the midgut. We envision our chemical probes will aid in the development of new selective mosquito control methods, thus preventing the spread of mosquito-borne illnesses with minimal impact on other organisms in the ecosystem.

Original languageEnglish (US)
Pages (from-to)8480-8485
Number of pages6
JournalJournal of the American Chemical Society
Volume146
Issue number12
DOIs
StatePublished - Mar 27 2024

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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