@article{5492a4342b614e6d98d15e300953972a,
title = "Chemical Labeling and Enrichment of Histone Glyoxal Adducts",
abstract = "Because of their long half-lives and highly nucleophilic tails, histones are particularly susceptible to accumulating nonenzymatic covalent modifications, such as glycation. The resulting modifications can have profound effects on cellular physiology due to the regulatory role histones play in all DNA-templated processes; however, the complexity of Maillard chemistry on proteins makes tracking and enriching for glycated proteins a challenging task. Here, we characterize glyoxal (GO) modifications on histones using quantitative proteomics and an aniline-derived GO-reactive probe. In addition, we leverage this chemistry to demonstrate that the glycation regulatory proteins DJ-1 and GLO1 reduce levels of histone GO adducts. Finally, we employ a two-round pull-down method to enrich histone H3 GO glycation and map these adducts to specific chromatin regions. ",
author = "Ray, {Devin M.} and Jennings, {Erin Q.} and Igor Maksimovic and Xander Chai and Galligan, {James J.} and Yael David and Qingfei Zheng",
note = "Funding Information: We extend our gratitude to the members of the David and Galligan laboratories for their invaluable support. We especially thank N. Prescott for qPCR technical assistance R. Pihl for idea refinement. Work in the David lab is supported by the Josie Robertson Foundation, the Pershing Square Sohn Cancer Research Alliance, the NIH (CCSG core Grant No. P30 CA008748, MSK SPORE P50 CA192937, and R35 GM138386), the Parker Institute for Cancer Immunotherapy, the STARR Cancer Alliance award, and the Anna Fuller Trust. In addition, the David lab is supported by W. H. Goodwin, A. Goodwin, and the Commonwealth Foundation for Cancer Research and the Center for Experimental Therapeutics at MSKCC. J.G. is supported by R35 GM137910, and Q.Z. is supported by the OSUCCC start-up funds. D.M.R. is supported by an MSTP grant from the National Institute of General Sciences of the NIH (T32 GM007739) to the Weill Cornell/Rockefeller/Sloan Kettering Tri-Institutional MD-PhD Program. E.J. is supported by NIH training Grant No. T32 ES007091. Publisher Copyright: {\textcopyright} 2022 American Chemical Society.",
year = "2021",
doi = "10.1021/acschembio.1c00864",
language = "English (US)",
journal = "ACS Chemical Biology",
issn = "1554-8929",
publisher = "American Chemical Society",
}