Chemical estimation of interstitial and basement membrane collagen in obstructive nephropathy

Ming Man; Ray B. Nagle; Elijah Adams

doi:10.1016/0014-4800(78)90034-5

Chemical estimation of interstitial and basement membrane collagen in obstructive nephropathy

Ming Man, Ray B. Nagle, Elijah Adams

Cancer Center

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8 Scopus citations

Abstract

Analyses of total 3-hydroxyproline and 4-hydroxyproline in tissue samples can be used to estimate the quantity of two kinds of collagen: that of basement-membrane and that of the several interstitial Types. Applied to an experimental model for obstructive nephropathy in the rabbit, such data show that the large increase in collagen in the renal cortex (at the 32nd day following unilateral ureteral ligation) is primarily of interstitial type. However there is also an appreciable increase in basement membrane collagen. Both findings are in agreement with the qualitative impression from electronmicrographs. The chemical method offers an approach to quantitation of these two kinds of collagen (basement membrane and interestitial) in normal and diseased tissues.

Original language	English (US)
Pages (from-to)	144-148
Number of pages	5
Journal	Experimental and Molecular Pathology
Volume	29
Issue number	2
DOIs	https://doi.org/10.1016/0014-4800(78)90034-5
State	Published - Oct 1978

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Clinical Biochemistry

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title = "Chemical estimation of interstitial and basement membrane collagen in obstructive nephropathy",

abstract = "Analyses of total 3-hydroxyproline and 4-hydroxyproline in tissue samples can be used to estimate the quantity of two kinds of collagen: that of basement-membrane and that of the several interstitial Types. Applied to an experimental model for obstructive nephropathy in the rabbit, such data show that the large increase in collagen in the renal cortex (at the 32nd day following unilateral ureteral ligation) is primarily of interstitial type. However there is also an appreciable increase in basement membrane collagen. Both findings are in agreement with the qualitative impression from electronmicrographs. The chemical method offers an approach to quantitation of these two kinds of collagen (basement membrane and interestitial) in normal and diseased tissues.",

author = "Ming Man and Nagle, {Ray B.} and Elijah Adams",

note = "Funding Information: 1 Supported by NIH Grant 2 Present address, Organic 3 Present address, Department 4 To whom reprint requests",

year = "1978",

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doi = "10.1016/0014-4800(78)90034-5",

language = "English (US)",

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journal = "Experimental and Molecular Pathology",

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T1 - Chemical estimation of interstitial and basement membrane collagen in obstructive nephropathy

AU - Man, Ming

AU - Nagle, Ray B.

AU - Adams, Elijah

N1 - Funding Information: 1 Supported by NIH Grant 2 Present address, Organic 3 Present address, Department 4 To whom reprint requests

PY - 1978/10

Y1 - 1978/10

N2 - Analyses of total 3-hydroxyproline and 4-hydroxyproline in tissue samples can be used to estimate the quantity of two kinds of collagen: that of basement-membrane and that of the several interstitial Types. Applied to an experimental model for obstructive nephropathy in the rabbit, such data show that the large increase in collagen in the renal cortex (at the 32nd day following unilateral ureteral ligation) is primarily of interstitial type. However there is also an appreciable increase in basement membrane collagen. Both findings are in agreement with the qualitative impression from electronmicrographs. The chemical method offers an approach to quantitation of these two kinds of collagen (basement membrane and interestitial) in normal and diseased tissues.

AB - Analyses of total 3-hydroxyproline and 4-hydroxyproline in tissue samples can be used to estimate the quantity of two kinds of collagen: that of basement-membrane and that of the several interstitial Types. Applied to an experimental model for obstructive nephropathy in the rabbit, such data show that the large increase in collagen in the renal cortex (at the 32nd day following unilateral ureteral ligation) is primarily of interstitial type. However there is also an appreciable increase in basement membrane collagen. Both findings are in agreement with the qualitative impression from electronmicrographs. The chemical method offers an approach to quantitation of these two kinds of collagen (basement membrane and interestitial) in normal and diseased tissues.

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Chemical estimation of interstitial and basement membrane collagen in obstructive nephropathy

Abstract

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