Characterizing brain metabolic function ex vivo with acute mouse slice punches

Guoyuan Qi, Yashi Mi, Fei Yin

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Mitochondrial dysfunction and metabolic reprogramming are implicated in a variety of neurological disorders. Here, we present a protocol that enables complex profiling of brain metabolic function using acute mouse brain slices ex vivo. Utilizing differential metabolic conditions, substrates, and inhibitors, this protocol can be broadly applied to determine metabolic shift or reprogramming upon genetic manipulations, pathological insults, or therapeutic interventions and could thus further the understanding of the dynamic role of energy metabolism in brain physiological function and diseases. For complete details on the use and execution of this protocol, please refer to Qi et al. (2021).

Original languageEnglish (US)
Article number100559
JournalSTAR Protocols
Issue number2
StatePublished - Jun 18 2021


  • Metabolism
  • Neuroscience

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine


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