Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications

Timothy S. Frost, Linan Jiang, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.

Original languageEnglish (US)
Title of host publicationProceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018
PublisherIEEE Computer Society
Pages27-30
Number of pages4
ISBN (Electronic)9781538675786
DOIs
StatePublished - Jul 2 2018
Event12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 - Waikiki Beach, United States
Duration: Dec 2 2018Dec 5 2018

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Volume2018-December
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

Conference12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018
Country/TerritoryUnited States
CityWaikiki Beach
Period12/2/1812/5/18

Keywords

  • A549 cell line
  • Convection-diffusion flow
  • Pharmacokinetics
  • Protein transport

ASJC Scopus subject areas

  • Molecular Medicine
  • Biomedical Engineering
  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications'. Together they form a unique fingerprint.

Cite this