TY - JOUR
T1 - Characterization of the molecular forms of ANP released by perfused neonatal rat heart
AU - Shields, Paul P.
AU - Glembotski, Christopher C.
N1 - Funding Information:
lsuuuorted Bv: This work was supported by NIH Grant NS25037 and American Heart Association Grant-in-Aid 84653 (to C.C.G.) and Grants RR-04-1412, RR-05415 and RR-07083 to the University of Pennsylvania Protein Chemistry Laboratory. C.C.G. is an Established Investigator of the American Heart Association. P.P.S. is supported by an NIH Cell and Molecular Biology Predoctoral Training Grant GM-07229.
PY - 1987/7/31
Y1 - 1987/7/31
N2 - Although cultures of neonatal rat atria and ventricles have been widely used to study ANP biosynthesis and secretion, little is known regarding the circulating form of ANP in neonatal animals. To begin to address this issue, we have developed a method for perfusing isolated neonatal rat hearts. Reversed phase-HPLC analysis of the heart effluents coupled with ANP RIA demonstrated that the predominant form of ANP released was chromatographically identical to ANP(99-126). Size exclusion-HPLC confirmed that the secreted ANP was indistinguishable from ANP(99-126). This demonstrated that the neonatal rat heart can efficiently generate and secrete a peptide similar to the circulating form of ANP found in adult rats, and further justifies the use of neonatal rat atria as a source of primary cells for studies of ANP biosynthesis and secretion.
AB - Although cultures of neonatal rat atria and ventricles have been widely used to study ANP biosynthesis and secretion, little is known regarding the circulating form of ANP in neonatal animals. To begin to address this issue, we have developed a method for perfusing isolated neonatal rat hearts. Reversed phase-HPLC analysis of the heart effluents coupled with ANP RIA demonstrated that the predominant form of ANP released was chromatographically identical to ANP(99-126). Size exclusion-HPLC confirmed that the secreted ANP was indistinguishable from ANP(99-126). This demonstrated that the neonatal rat heart can efficiently generate and secrete a peptide similar to the circulating form of ANP found in adult rats, and further justifies the use of neonatal rat atria as a source of primary cells for studies of ANP biosynthesis and secretion.
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U2 - 10.1016/0006-291X(87)90563-8
DO - 10.1016/0006-291X(87)90563-8
M3 - Article
C2 - 2956946
AN - SCOPUS:0023669176
SN - 0006-291X
VL - 146
SP - 547
EP - 553
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -