Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant

David DeShazer; Jason D. Barnnan; Michael J. Moran; Richard L. Friedman

doi:10.1016/0378-1119(94)90359-X

Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant

David DeShazer
, Jason D. Barnnan
, Michael J. Moran
, Richard L. Friedman

Immunobiology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe⁺ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.

Original language	English (US)
Pages (from-to)	85-89
Number of pages	5
Journal	Gene
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)90359-X
State	Published - May 3 1994

Keywords

Oxidative stress
homologous recombination
human pathogen
insertion mutagenesis
metalloenzyme
suicide vector

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(94)90359-X

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title = "Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant",

abstract = "The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe+ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.",

keywords = "Oxidative stress, homologous recombination, human pathogen, insertion mutagenesis, metalloenzyme, suicide vector",

author = "David DeShazer and Barnnan, \{Jason D.\} and Moran, \{Michael J.\} and Friedman, \{Richard L.\}",

year = "1994",

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doi = "10.1016/0378-1119(94)90359-X",

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T1 - Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant

AU - DeShazer, David

AU - Barnnan, Jason D.

AU - Moran, Michael J.

AU - Friedman, Richard L.

PY - 1994/5/3

Y1 - 1994/5/3

N2 - The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe+ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.

AB - The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe+ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.

KW - Oxidative stress

KW - homologous recombination

KW - human pathogen

KW - insertion mutagenesis

KW - metalloenzyme

KW - suicide vector

UR - https://www.scopus.com/pages/publications/0028269890

UR - https://www.scopus.com/pages/publications/0028269890#tab=citedBy

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DO - 10.1016/0378-1119(94)90359-X

M3 - Article

C2 - 8181762

AN - SCOPUS:0028269890

SN - 0378-1119

VL - 142

SP - 85

EP - 89

JO - Gene

JF - Gene

IS - 1

ER -

Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant

Abstract

Keywords

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