@article{f31e5a8932d54875a58a5ccd0baf9029,
title = "Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid",
abstract = "Leucine-rich repeat-containing G-protein coupled receptor 5-expressing (Lgr5+) cells have been identified as stem/progenitor cells in the circumvallate papillae, and single cultured Lgr5+ cells give rise to taste cells. Here we use circumvallate papilla tissue to establish a three-dimensional culture system (taste bud organoids) that develops phenotypic characteristics similar to native tissue, including a multilayered epithelium containing stem/progenitor in the outer layers and taste cells in the inner layers. Furthermore, characterization of the cell cycle of the taste bud progenitor niche reveals striking dynamics of taste bud development and regeneration. Using this taste bud organoid culture system and FUCCI2 transgenic mice, we identify the stem/progenitor cells have at least 5 distinct cell cycle populations by tracking within 24-hour synchronized oscillations of proliferation. Additionally, we demonstrate that stem/progenitor cells have motility to form taste bud organoids. Taste bud organoids provides a system for elucidating mechanisms of taste signaling, disease modeling, and taste tissue regeneration.",
author = "Eitaro Aihara and Mahe, {Maxime M.} and Schumacher, {Michael A.} and Matthis, {Andrea L.} and Rui Feng and Wenwen Ren and Noah, {Taeko K.} and Toru Matsu-Ura and Moore, {Sean R.} and Hong, {Christian I.} and Yana Zavros and Scott Herness and Shroyer, {Noah F.} and Ken Iwatsuki and Peihua Jiang and Helmrath, {Michael A.} and Montrose, {Marshall H.}",
note = "Funding Information: We thank RIKEN for providing R26p-FUCCI2 and R26-H2B-EGFP mice, and Drs. Takaya Abe, Go Shioi, and Hiroshi Kiyonari (RIKEN Center for Developmental Biology, Japan) for their technical support. We also gratefully acknowledge Dr. Hans Clevers (Hubrecht Institute for Developmental Biology and Stem Cell Research, Netherlands) for the kind gift of Wnt secreting L-cells. We also acknowledge Dr. Jeffrey Whitsett for kindly donating the modified R-spondin secreting HEK-293T cells (Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati Children{\textquoteright}s Hospital Medical Center and The University of Cincinnati College of Medicine, Cincinnati, OH). This project was supported in part by Department of Interior Grant D12AP00005 (S.R.M and C.I.H.) and NIH P30 DK078392 (Integrative Morphology Core of the Digestive Disease Research Core Center in Cincinnati). Publisher Copyright: {\textcopyright} 2015, Nature Publishing Group. All rights reserved.",
year = "2015",
month = nov,
day = "24",
doi = "10.1038/srep17185",
language = "English (US)",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}