Characterization of stem/progenitor cell cycle using murine circumvallate papilla taste bud organoid

Eitaro Aihara, Maxime M. Mahe, Michael A. Schumacher, Andrea L. Matthis, Rui Feng, Wenwen Ren, Taeko K. Noah, Toru Matsu-Ura, Sean R. Moore, Christian I. Hong, Yana Zavros, Scott Herness, Noah F. Shroyer, Ken Iwatsuki, Peihua Jiang, Michael A. Helmrath, Marshall H. Montrose

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Leucine-rich repeat-containing G-protein coupled receptor 5-expressing (Lgr5+) cells have been identified as stem/progenitor cells in the circumvallate papillae, and single cultured Lgr5+ cells give rise to taste cells. Here we use circumvallate papilla tissue to establish a three-dimensional culture system (taste bud organoids) that develops phenotypic characteristics similar to native tissue, including a multilayered epithelium containing stem/progenitor in the outer layers and taste cells in the inner layers. Furthermore, characterization of the cell cycle of the taste bud progenitor niche reveals striking dynamics of taste bud development and regeneration. Using this taste bud organoid culture system and FUCCI2 transgenic mice, we identify the stem/progenitor cells have at least 5 distinct cell cycle populations by tracking within 24-hour synchronized oscillations of proliferation. Additionally, we demonstrate that stem/progenitor cells have motility to form taste bud organoids. Taste bud organoids provides a system for elucidating mechanisms of taste signaling, disease modeling, and taste tissue regeneration.

Original languageEnglish (US)
Article number17185
JournalScientific reports
Volume5
DOIs
StatePublished - Nov 24 2015
Externally publishedYes

ASJC Scopus subject areas

  • General

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