TY - JOUR
T1 - Characterization of sodium channel α- and β-subunits in rat and mouse cardiac myocytes
AU - Malhotra, Jyoti Dhar
AU - Chen, Chunling
AU - Rivolta, Ilaria
AU - Abriel, Hugues
AU - Malhotra, Ricky
AU - Mattei, Laura N.
AU - Brosius, Frank C.
AU - Kass, Robert S.
AU - Isom, Lori L.
PY - 2001/3/6
Y1 - 2001/3/6
N2 - Background - Sodium channels isolated from mammalian brain are composed of α-, β1-, and β2-subunits. The composition of sodium channels in cardiac muscle, however, has not been defined, and disagreement exists over which β-subunits are expressed in the myocytes. Some investigators have demonstrated β1 expression in heart. Others have not detected any auxiliary subunits. On the basis of Northern blot analysis of total RNA, β2 expression has been thought to be exclusive to neurons and absent from cardiac muscle. Methods and Results - The goal of this study was to define the subunit composition of cardiac sodium channels in myocytes. We show that cardiac sodium channels are composed of α-, β1-, and β2-subunits. Nav1.5 and Nav1.1 are expressed in myocytes and are associated with β1- and β2-subunits. Immunocytochemical localization of Nav1.1, β1, and β2 in adult heart sections showed that these subunits are expressed at the Z lines, as shown previously for Nav1.5. Coexpression of Nav1.5 with β2 in transfected cells resulted in no detectable changes in sodium current. Conclusions - Cardiac sodium channels are composed of α- (Nav1.1 or Nav1.5), β1-, and β2-subunits. Although β1-subunits modulate cardiac sodium channel current, β2-subunit function in heart may be limited to cell adhesion.
AB - Background - Sodium channels isolated from mammalian brain are composed of α-, β1-, and β2-subunits. The composition of sodium channels in cardiac muscle, however, has not been defined, and disagreement exists over which β-subunits are expressed in the myocytes. Some investigators have demonstrated β1 expression in heart. Others have not detected any auxiliary subunits. On the basis of Northern blot analysis of total RNA, β2 expression has been thought to be exclusive to neurons and absent from cardiac muscle. Methods and Results - The goal of this study was to define the subunit composition of cardiac sodium channels in myocytes. We show that cardiac sodium channels are composed of α-, β1-, and β2-subunits. Nav1.5 and Nav1.1 are expressed in myocytes and are associated with β1- and β2-subunits. Immunocytochemical localization of Nav1.1, β1, and β2 in adult heart sections showed that these subunits are expressed at the Z lines, as shown previously for Nav1.5. Coexpression of Nav1.5 with β2 in transfected cells resulted in no detectable changes in sodium current. Conclusions - Cardiac sodium channels are composed of α- (Nav1.1 or Nav1.5), β1-, and β2-subunits. Although β1-subunits modulate cardiac sodium channel current, β2-subunit function in heart may be limited to cell adhesion.
KW - Cells
KW - Genes
KW - Ion channels
KW - Signal transduction
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U2 - 10.1161/01.CIR.103.9.1303
DO - 10.1161/01.CIR.103.9.1303
M3 - Article
C2 - 11238277
AN - SCOPUS:0035814946
SN - 0009-7322
VL - 103
SP - 1303
EP - 1310
JO - Circulation
JF - Circulation
IS - 9
ER -