Characterization of Giardia lamblia genotypes in dogs from Tucson, Arizona using SSU-rRNA and β-giardin sequences

K. M. Johansen, N. S. Castro, K. E. Lancaster, E. Madrid, A. Havas, J. Simms, C. R. Sterling

    Research output: Contribution to journalArticlepeer-review

    16 Scopus citations


    The objective of this study was to determine if human genotypes of Giardia lamblia could be found in canine companion animals from urban and peri-urban environments in Tucson, Arizona. Canine fecal samples collected from the Humane Society of Southern Arizona between July 2006 and April 2009 were screened for G. lamblia infection using immunofluorescent microscopy and confirmed by polymerase chain reaction (PCR). Of the 672 samples screened, 196 were found positive by IFA and 185 of those positive were successfully amplified through PCR. Sequencing analysis showed samples were primarily of the C or D genotypes (n = 154), or showing a mix of the C and D genotypes (n = 10). One sample showed a mixed infection of a human genotype (A) and a dog-specific genotype (C). These data are consistent with previous studies showing dog specific genotypes to be dominant in environments where dog-to-dog transmission is likely to occur, and provides further evidence that multiple genes should be targeted for more accurate genotype characterization.

    Original languageEnglish (US)
    Pages (from-to)387-390
    Number of pages4
    JournalParasitology Research
    Issue number1
    StatePublished - Jan 2014

    ASJC Scopus subject areas

    • Parasitology
    • General Veterinary
    • Insect Science
    • Infectious Diseases


    Dive into the research topics of 'Characterization of Giardia lamblia genotypes in dogs from Tucson, Arizona using SSU-rRNA and β-giardin sequences'. Together they form a unique fingerprint.

    Cite this