TY - JOUR
T1 - Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their expression in rapidly dividing tissues
AU - Woo, Ho Hyung
AU - Jeong, Byeong Ryong
AU - Hirsch, Ann M.
AU - Hawes, Martha C.
N1 - Funding Information:
The project was supported in part by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (Grant 2003-35304-13362) and the Department of Energy (Grant defg0394er20164, to MCH and H.-H.W., by NIH/NCCAMSPSO AT00151 to the Center for Dietary Supplement Research Botanicals at UCLA (A.M.H., Director, Agricultural Botany Core and H.-H.W., Junior Investigator), and by a grant from the Shanbrom Family Foundation to A.M.H. B.R.J. was supported in part by Daegu University Research Grant, 2004, and in part by Daegu University RRC Program. All plasmids and transgenic plant seeds will be available at the Ohio State University Arabidopsis stock center.
PY - 2007/7
Y1 - 2007/7
N2 - In humans, uridine 5′-diphosphate glucuronosyltransferase (UGT) operates in opposition to glucuronidase (GUS) to control activity of diverse metabolites such as hormones by reversible conjugation with glucuronic acid. Previous data revealed that, as in mammals, these enzymes are required for plant life in that a UGT from Pisum sativum (PsUGT1) controls plant development by opposing endogenous GUS activity thereby modulating the duration of the cell cycle. Here we report that a small family of genes (AtUGT85A1, 2, 3, 4, 5, and 7) homologous to pea PsUGT1 exists in the Arabidopsis genome. The AtUGT85A-encoded proteins are predicted to be membrane-associated enzymes. Three genes (AtGUS1, AtGUS2, and AtGUS3) that are homologous to a GUS-encoding gene from Scutellaria baicalensis were identified. The AtGUS-encoded proteins are predicted to be secretory (AtGUS1) and membrane-associated (AtGUS2 and AtGUS3) enzymes. Both AtUGT85A and AtGUS genes, like PsUGT1, exhibit localized, tissue-specific expression, mainly in areas of active cell division with possible involvement in cell cycle regulation.
AB - In humans, uridine 5′-diphosphate glucuronosyltransferase (UGT) operates in opposition to glucuronidase (GUS) to control activity of diverse metabolites such as hormones by reversible conjugation with glucuronic acid. Previous data revealed that, as in mammals, these enzymes are required for plant life in that a UGT from Pisum sativum (PsUGT1) controls plant development by opposing endogenous GUS activity thereby modulating the duration of the cell cycle. Here we report that a small family of genes (AtUGT85A1, 2, 3, 4, 5, and 7) homologous to pea PsUGT1 exists in the Arabidopsis genome. The AtUGT85A-encoded proteins are predicted to be membrane-associated enzymes. Three genes (AtGUS1, AtGUS2, and AtGUS3) that are homologous to a GUS-encoding gene from Scutellaria baicalensis were identified. The AtGUS-encoded proteins are predicted to be secretory (AtGUS1) and membrane-associated (AtGUS2 and AtGUS3) enzymes. Both AtUGT85A and AtGUS genes, like PsUGT1, exhibit localized, tissue-specific expression, mainly in areas of active cell division with possible involvement in cell cycle regulation.
KW - Arabidopsis AtUGT85A family
KW - Glucuronidase
KW - Lethality
KW - UDP-glycosyltransferase
UR - http://www.scopus.com/inward/record.url?scp=34248198906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34248198906&partnerID=8YFLogxK
U2 - 10.1016/j.ygeno.2007.03.014
DO - 10.1016/j.ygeno.2007.03.014
M3 - Article
C2 - 17498920
AN - SCOPUS:34248198906
SN - 0888-7543
VL - 90
SP - 143
EP - 153
JO - Genomics
JF - Genomics
IS - 1
ER -