Central role of the PPARγ gene network in coordinating beef cattle intramuscular adipogenesis in response to weaning age and nutrition

Sonia J. Moisá, Daniel W. Shike, Dan B. Faulkner, William T. Meteer, Duane Keisler, Juan J. Loor

    Research output: Contribution to journalArticlepeer-review

    58 Scopus citations

    Abstract

    Adipogenic/lipogenic transcriptional networks regulating intramuscular fat deposition (IMF) in response to weaning age and dietary starch level were studied. The longissimus muscle (LM) of beef steers on an early weaning (141 days age) plus high-starch diet (EWS) or a normal weaning (NW, 222 days age) plus starch creep-feed diet (CFS) was biopsied at 0 (EW), 25, 50, 96 (NW), 167, and 222 (pre-slaughter) days. Expression patterns of 35 target genes were studied. From NW through slaughter, all steers received the same high-starch diet. In EWS steers the expression of PPARG, other adipogenic (CEBPA, ZFP423) and lipogenic (THRSP, SREBF1, INSIG1) activators, and several enzymes (FASN, SCD, ELOVL6, PCK1, DGAT2) that participate in the process of IMF increased gradually to a peak between 96 and 167 days on treatment. Steers in NW did not achieve similar expression levels even by 222 days on treatment, suggesting a blunted response even when fed a high-starch diet after weaning. High-starch feeding at an early age (EWS) triggers precocious and sustained adipogenesis, resulting in greater marbling.

    Original languageEnglish (US)
    Pages (from-to)17-32
    Number of pages16
    JournalGene Regulation and Systems Biology
    Volume2014
    Issue number8
    DOIs
    StatePublished - Jan 8 2014

    Keywords

    • Adipogenesis
    • Marbling
    • Nutrition
    • Transcriptomics
    • Weaning

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Molecular Biology
    • Genetics
    • Computer Science Applications

    Fingerprint

    Dive into the research topics of 'Central role of the PPARγ gene network in coordinating beef cattle intramuscular adipogenesis in response to weaning age and nutrition'. Together they form a unique fingerprint.

    Cite this