Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam

Dongli Xu; Weibin Zhou; Leilei Peng

doi:10.1364/BOE.8.000570

Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam

Dongli Xu, Weibin Zhou, Leilei Peng

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.

Original language	English (US)
Article number	#275798
Pages (from-to)	570-578
Number of pages	9
Journal	Biomedical Optics Express
Volume	8
Issue number	2
DOIs	https://doi.org/10.1364/BOE.8.000570
State	Published - Feb 1 2017

Keywords

Fluorescence microscopy
Imaging systems
Spectroscopy, fluorescence and luminescence
Three-dimensional microscopy

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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10.1364/BOE.8.000570

Cite this

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title = "Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam",

abstract = "Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.",

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AU - Zhou, Weibin

AU - Peng, Leilei

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AB - Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.

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KW - Spectroscopy, fluorescence and luminescence

KW - Three-dimensional microscopy

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Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam

Abstract

Keywords

ASJC Scopus subject areas

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