Cellular immune responses in amniotic fluid of women with preterm prelabor rupture of membranes

Jose Galaz, Roberto Romero, Rebecca Slutsky, Yi Xu, Kenichiro Motomura, Robert Para, Percy Pacora, Bogdan Panaitescu, Chaur Dong Hsu, Marian Kacerovsky, Nardhy Gomez-Lopez

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Preterm birth is the leading cause of perinatal morbidity and mortality. Preterm prelabor rupture of membranes (pPROM) occurs in 30% of preterm births; thus, this complication is a major contributor to maternal and neonatal morbidity. However, the cellular immune responses in amniotic fluid of women with pPROM have not been investigated. Amniotic fluid samples were obtained from women with pPROM and a positive (n = 7) or negative (n = 10) microbiological culture. Flow cytometry was performed to evaluate the phenotype and number of amniotic fluid leukocytes. The correlation between amniotic fluid immune cells and an interleukin-6 (IL-6) concentration or a white blood cell (WBC) count in amniotic fluid was calculated. Women with pPROM and a positive amniotic fluid culture had (1) a greater number of total leukocytes in amniotic fluid, including neutrophils and monocytes/macrophages and (2) an increased number of total T cells in amniotic fluid, namely CD4+ T cells and CD8+ T cells, but not B cells. The numbers of neutrophils and monocytes/macrophages were positively correlated with IL-6 concentrations and WBC counts in amniotic fluid of women with pPROM. Women with pPROM and a positive amniotic fluid culture exhibit a more severe cellular immune response than those with a negative culture, which is associated with well-known markers of intra-amniotic inflammation.

Original languageEnglish (US)
Pages (from-to)222-233
Number of pages12
JournalJournal of Perinatal Medicine
Volume48
Issue number3
DOIs
StatePublished - Mar 1 2020
Externally publishedYes

Keywords

  • acute chorioamnionitis
  • clinical chorioamnionitis
  • fetal inflammatory response
  • funisitis
  • innate immune cells
  • interleukin-6
  • labor
  • microbial invasion of the amniotic cavity
  • pregnancy
  • prematurity
  • preterm labor

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Fingerprint

Dive into the research topics of 'Cellular immune responses in amniotic fluid of women with preterm prelabor rupture of membranes'. Together they form a unique fingerprint.

Cite this