Cellular immune responses in amniotic fluid of women with preterm clinical chorioamnionitis

Jose Galaz, Roberto Romero, Yi Xu, Derek Miller, Rebecca Slutsky, Dustyn Levenson, Chaur Dong Hsu, Nardhy Gomez-Lopez

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Objective: Preterm birth is the leading cause of neonatal morbidity and mortality worldwide. Some preterm births are associated with clinical chorioamnionitis; yet, this condition has been poorly investigated. Herein, we characterized the amniotic fluid cellular immune responses in women with preterm clinical chorioamnionitis. Methods and subjects: Amniotic fluid samples were obtained from women with preterm clinical chorioamnionitis and a positive or negative microbiological culture (n = 17). The cellular composition of amniotic fluid was evaluated using fluorescence microscopy, scanning and transmission electron microscopy, and flow cytometry. Women without preterm clinical chorioamnionitis were also examined (n = 10). Results: Amniotic fluid from women with preterm clinical chorioamnionitis and a positive culture had: (1) abundant neutrophils associated with viable and non-viable bacteria, (2) neutrophils performing phagocytosis, (3) neutrophils forming NETs, (4) increased numbers of neutrophils, monocytes/macrophages, and CD4+ T cells, and (5) high expression of IL-1β by neutrophils and monocytes/macrophages. Amniotic fluid from women with preterm clinical chorioamnionitis and proven infection tended to have fewer monocytes/macrophages and CD4+ T cells compared to those without chorioamnionitis. Conclusion: We provide the first morphologic and phenotypic characterization of the cellular immune responses in the amniotic cavity of women with preterm clinical chorioamnionitis, a condition associated with adverse neonatal outcomes.

Original languageEnglish (US)
Pages (from-to)203-216
Number of pages14
JournalInflammation Research
Volume69
Issue number2
DOIs
StatePublished - Feb 1 2020
Externally publishedYes

Keywords

  • Acute chorioamnionitis
  • Immune Cells
  • Immunology
  • Macrophages
  • Monocytes
  • Neutrophils
  • T Cells

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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