Cellular immune responses in amniotic fluid of women with a sonographic short cervix

Jose Galaz, Roberto Romero, Yi Xu, Derek Miller, Dustyn Levenson, Robert Para, Aneesha Varrey, Richard Hsu, Anna Tong, Sonia S. Hassan, Chaur Dong Hsu, Nardhy Gomez-Lopez

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Objectives: A sonographic short cervix is one of the strongest predictors of preterm delivery. However, the cellular immune composition of amniotic fluid in women with a short cervix has not yet been described. Herein, we determined cellular and soluble immune responses in amniotic fluid from pregnant women with a mid-trimester asymptomatic short cervix. Methods: Amniotic fluid samples (n=77) were collected from asymptomatic women with a cervical length between 15 and 25 mm (n=36, short cervix) or ≤15 mm (n=41, severely short cervix) diagnosed by ultrasound. Flow cytometry and multiplex measurement of cytokines/chemokines were performed. Results: (1) The cellular immune composition of amniotic fluid did not differ between women with a severely short cervix (≤15 mm) and those with a short cervix 15-25 mm; (2) amniotic fluid concentrations of multiple cytokines/chemokines were higher in women with a severely short cervix (≤15 mm) than in those with a short cervix 15-25 mm; (3) the cellular immune composition of amniotic fluid did not differ between women with a severely short cervix (≤15 mm) who ultimately underwent preterm delivery and those who delivered at term; and (4) amniotic fluid concentrations of IL-2, but not other immune mediators, were increased in women with a severely short cervix (≤15 mm) who ultimately delivered preterm compared to those who delivered at term. Conclusions: Women with a severely short cervix (≤15 mm) have increased concentrations of pro-inflammatory mediators in the amniotic cavity; yet, these do not translate to changes in the cellular immune response.

Original languageEnglish (US)
Pages (from-to)665-676
Number of pages12
JournalJournal of Perinatal Medicine
Volume48
Issue number7
DOIs
StatePublished - Sep 1 2020
Externally publishedYes

Keywords

  • Amniotic fluid
  • Chemokine
  • Cytokine
  • Immune cell
  • Intra-amniotic inflammation
  • Preterm

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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