Abstract
The hormonally inactive methyl-3,5-diiodo-4-(4′-methoxyphenoxy) benzoate (DIME) at 1-4 μM concentration induces morphologic changes in E-ras 20 and MDA-MB-231 and other human cancer cells such as multinucleation and enlargement and arrests the cell cycle in the M phase without affecting interphase. Time-lapse videomicroscopy allowed us to follow individual cells. Cells exposed to DIME divided in an abnormal manner, leading to 20% cell fusion and multinucleation. Chromosome painting demonstrated a large accumulation of chromosomes after 5 days of treatment with DIME, consistent with the failure of cells to divide normally. Chromosome breakage was not observed. On the other hand, highly abnormal tubulin-containing structures ensue upon exposure to DIME (1 μM for 18 h) treatment, indicating an early biochemical action of DIME on the spindle assembly system.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 905-910 |
| Number of pages | 6 |
| Journal | International journal of oncology |
| Volume | 10 |
| Issue number | 5 |
| State | Published - 1997 |
Keywords
- Cell division
- Flow cytometry
- Time-lapse videomicroscopy
- Tubulin
ASJC Scopus subject areas
- Oncology
- Cancer Research