TY - JOUR
T1 - Cellular accumulation of dietary anticarcinogenic isothiocyanates is followed by transporter-mediated export as dithiocarbamates
AU - Callaway, Eileen C.
AU - Zhang, Yuesheng
AU - Chew, Wade
AU - Chow, H. H.Sherry
N1 - Funding Information:
This research was supported in part by RO1 grant CA80962 from the National Cancer Institute, National Institutes of Health. The LC/MS/MS experiments were carried out in the Analytical Core Shared Service of the Arizona Cancer Center (CA23074). We thank Bradley C. Helbing for assistance in the preparation of this manuscript.
PY - 2004/2/10
Y1 - 2004/2/10
N2 - Many dietary isothiocyanates (ITCs) are potent anticarcinogenic agents. ITCs rapidly accumulate to high concentrations in cells as a result of conjugation with intracellular thiols, especially glutathione (GSH). The anticarcinogenic activity of ITCs depends on, at least partly, their accumulation in cells. We report that three major anticarcinogenic ITCs, including allyl-ITC, benzyl-ITC, and phenethyl-ITC, were rapidly exported, upon accumulation in cells, mainly in the forms of GSH- and cysteinylglycine- conjugates, apparently involving MRP-1 and Pgp-1. These findings are consistent with our previous results regarding cellular export of another anticarcinogenic ITC, sulforaphane, and suggest a common cellular response to ITCs.
AB - Many dietary isothiocyanates (ITCs) are potent anticarcinogenic agents. ITCs rapidly accumulate to high concentrations in cells as a result of conjugation with intracellular thiols, especially glutathione (GSH). The anticarcinogenic activity of ITCs depends on, at least partly, their accumulation in cells. We report that three major anticarcinogenic ITCs, including allyl-ITC, benzyl-ITC, and phenethyl-ITC, were rapidly exported, upon accumulation in cells, mainly in the forms of GSH- and cysteinylglycine- conjugates, apparently involving MRP-1 and Pgp-1. These findings are consistent with our previous results regarding cellular export of another anticarcinogenic ITC, sulforaphane, and suggest a common cellular response to ITCs.
KW - Allyl isothiocyanate
KW - Benzyl isothiocyanate
KW - Isothiocyanate
KW - Isothiocyanate transport
KW - Multidrug resistance associated protein-1
KW - P-glycoprotein-1
KW - Phenethyl isothiocyanate
UR - http://www.scopus.com/inward/record.url?scp=1642523398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1642523398&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2003.09.021
DO - 10.1016/j.canlet.2003.09.021
M3 - Article
C2 - 14744531
AN - SCOPUS:1642523398
SN - 0304-3835
VL - 204
SP - 23
EP - 31
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -