Cell targeting peptide conjugation to siRNA polyplexes for effective gene silencing in cardiomyocytes

Hye Yeong Nam, Jaesung Kim, Sung Wan Kim, David A. Bull

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

To deliver siRNA specifically to cardiomyocytes with a high transfection efficiency, primary cardiomyocyte-targeting (PCM) and/or cell-penetrating (Tat) peptides were incorporated into the siRNA. With the addition of plasmid DNA, these peptide-conjugated siRNAs were able to form compact and stable nanosized polyplex particles with bioreducible poly(CBA-DAH). The peptide-modified siRNA polyplexes enhanced the cellular uptake and the gene-silencing capacity of the siRNA in cardiomyocytes without significant immunogenicity or cytotoxicity. These findings demonstrate that the cell-targeting peptide and/or cell-penetrating peptide conjugation of siRNA may be a potentially important strategy for cell-specific gene therapy in gene-mediated disease states.

Original languageEnglish (US)
Pages (from-to)1302-1309
Number of pages8
JournalMolecular Pharmaceutics
Volume9
Issue number5
DOIs
StatePublished - May 7 2012
Externally publishedYes

Keywords

  • bioreducible polymer
  • gene therapy
  • siRNA
  • targeting

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

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