Cell surface turnover and shedding of fucosyl-glycoprotein in L6 cells

Thomas C. Doetschman

doi:10.1016/0309-1651(80)90219-2

Cell surface turnover and shedding of fucosyl-glycoprotein in L6 cells

Thomas C. Doetschman

Cellular & Molecular Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The disappearance of electrophoretically identifiable ³H-fucosylated glycoproteins from the cell surface of L6 cells is random and follows monophasic kinetics with a half-life of 9 hours. Approximately 47% and 53% of that loss is due to shedding and to degradation, respectively. Shedding also occurs with monophasic kinetics. Most of the electrophoretically identifiable-shed fucosyl-glycoproteins are released with homogeneous rates but do not represent a random sampling of the surface.

Original language	English (US)
Pages (from-to)	379-390
Number of pages	12
Journal	Cell Biology International Reports
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/0309-1651(80)90219-2
State	Published - Apr 1980

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0309-1651(80)90219-2

Cite this

@article{0f97e744da274b3d912b63d2d94bb31d,

title = "Cell surface turnover and shedding of fucosyl-glycoprotein in L6 cells",

abstract = "The disappearance of electrophoretically identifiable 3H-fucosylated glycoproteins from the cell surface of L6 cells is random and follows monophasic kinetics with a half-life of 9 hours. Approximately 47\% and 53\% of that loss is due to shedding and to degradation, respectively. Shedding also occurs with monophasic kinetics. Most of the electrophoretically identifiable-shed fucosyl-glycoproteins are released with homogeneous rates but do not represent a random sampling of the surface.",

author = "Doetschman, \{Thomas C.\}",

note = "Funding Information: ACKNOWLEDGEMENTS. I wish to thank Dr. Heinz Herrmann (University of Connecticut) for his support and advise on all aspects of this work, and Ms. Donna Mayer (University of Connecticut) for critical readings0.E the manuscript. The \textasciitilde{}6 cell line was a gift from Dr. David Yaffe (Weizmann Institute, Israel), This research was benefitted from use of the Cell Culture Facility supported by National Cancer Institute Grant CCA14733. This research was supported by a Muscular Dystrophy Association Grant to Dr. Heinz Herrmann and by American Cancer Society Grant \#RDPB.",

year = "1980",

month = apr,

doi = "10.1016/0309-1651(80)90219-2",

language = "English (US)",

volume = "4",

pages = "379--390",

journal = "Cell Biology International Reports",

issn = "0309-1651",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Cell surface turnover and shedding of fucosyl-glycoprotein in L6 cells

AU - Doetschman, Thomas C.

N1 - Funding Information: ACKNOWLEDGEMENTS. I wish to thank Dr. Heinz Herrmann (University of Connecticut) for his support and advise on all aspects of this work, and Ms. Donna Mayer (University of Connecticut) for critical readings0.E the manuscript. The ~6 cell line was a gift from Dr. David Yaffe (Weizmann Institute, Israel), This research was benefitted from use of the Cell Culture Facility supported by National Cancer Institute Grant CCA14733. This research was supported by a Muscular Dystrophy Association Grant to Dr. Heinz Herrmann and by American Cancer Society Grant #RDPB.

PY - 1980/4

Y1 - 1980/4

N2 - The disappearance of electrophoretically identifiable 3H-fucosylated glycoproteins from the cell surface of L6 cells is random and follows monophasic kinetics with a half-life of 9 hours. Approximately 47% and 53% of that loss is due to shedding and to degradation, respectively. Shedding also occurs with monophasic kinetics. Most of the electrophoretically identifiable-shed fucosyl-glycoproteins are released with homogeneous rates but do not represent a random sampling of the surface.

AB - The disappearance of electrophoretically identifiable 3H-fucosylated glycoproteins from the cell surface of L6 cells is random and follows monophasic kinetics with a half-life of 9 hours. Approximately 47% and 53% of that loss is due to shedding and to degradation, respectively. Shedding also occurs with monophasic kinetics. Most of the electrophoretically identifiable-shed fucosyl-glycoproteins are released with homogeneous rates but do not represent a random sampling of the surface.

UR - https://www.scopus.com/pages/publications/0018819153

UR - https://www.scopus.com/pages/publications/0018819153#tab=citedBy

U2 - 10.1016/0309-1651(80)90219-2

DO - 10.1016/0309-1651(80)90219-2

M3 - Article

C2 - 7388976

AN - SCOPUS:0018819153

SN - 0309-1651

VL - 4

SP - 379

EP - 390

JO - Cell Biology International Reports

JF - Cell Biology International Reports

IS - 4

ER -

Cell surface turnover and shedding of fucosyl-glycoprotein in L6 cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this