Causes of retrograde flow in fish keratocytes

Thomas Fuhs; Michael Goegler; Claudia A. Brunner; Charles W. Wolgemuth; Josef A. Kaes

doi:10.1002/cm.21151

Causes of retrograde flow in fish keratocytes

Thomas Fuhs, Michael Goegler, Claudia A. Brunner, Charles W. Wolgemuth, Josef A. Kaes

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Confronting motile cells with obstacles doubling as force sensors we tested the limits of the driving actin and myosin machinery. We could directly measure the force necessary to stop actin polymerization as well as the force present in the retrograde actin flow. Combined with detailed measurements of the retrograde flow velocity and specific manipulation of actin and myosin we found that actin polymerization and myosin contractility are not enough to explain the cells behavior. We show that ever-present depolymerization forces, a direct entropic consequence of actin filament recycling, are sufficient to fill this gap, even under heavy loads.

Original language	English (US)
Pages (from-to)	24-35
Number of pages	12
Journal	Cytoskeleton
Volume	71
Issue number	1
DOIs	https://doi.org/10.1002/cm.21151
State	Published - Jan 2014

Keywords

Actin network and myosin motors
Atomic force microscopy
Cell motility and forces
Forces causing retrograde actin flow
Polymerization and depolymerization force

ASJC Scopus subject areas

Structural Biology
Cell Biology

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10.1002/cm.21151

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abstract = "Confronting motile cells with obstacles doubling as force sensors we tested the limits of the driving actin and myosin machinery. We could directly measure the force necessary to stop actin polymerization as well as the force present in the retrograde actin flow. Combined with detailed measurements of the retrograde flow velocity and specific manipulation of actin and myosin we found that actin polymerization and myosin contractility are not enough to explain the cells behavior. We show that ever-present depolymerization forces, a direct entropic consequence of actin filament recycling, are sufficient to fill this gap, even under heavy loads.",

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AU - Goegler, Michael

AU - Brunner, Claudia A.

AU - Wolgemuth, Charles W.

AU - Kaes, Josef A.

PY - 2014/1

Y1 - 2014/1

N2 - Confronting motile cells with obstacles doubling as force sensors we tested the limits of the driving actin and myosin machinery. We could directly measure the force necessary to stop actin polymerization as well as the force present in the retrograde actin flow. Combined with detailed measurements of the retrograde flow velocity and specific manipulation of actin and myosin we found that actin polymerization and myosin contractility are not enough to explain the cells behavior. We show that ever-present depolymerization forces, a direct entropic consequence of actin filament recycling, are sufficient to fill this gap, even under heavy loads.

AB - Confronting motile cells with obstacles doubling as force sensors we tested the limits of the driving actin and myosin machinery. We could directly measure the force necessary to stop actin polymerization as well as the force present in the retrograde actin flow. Combined with detailed measurements of the retrograde flow velocity and specific manipulation of actin and myosin we found that actin polymerization and myosin contractility are not enough to explain the cells behavior. We show that ever-present depolymerization forces, a direct entropic consequence of actin filament recycling, are sufficient to fill this gap, even under heavy loads.

KW - Actin network and myosin motors

KW - Atomic force microscopy

KW - Cell motility and forces

KW - Forces causing retrograde actin flow

KW - Polymerization and depolymerization force

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Causes of retrograde flow in fish keratocytes

Abstract

Keywords

ASJC Scopus subject areas

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