TY - JOUR
T1 - Catalysis of S-nitrosothiols formation by serum albumin
T2 - The mechanism and implication in vascular control
AU - Rafikova, Olga
AU - Rafikov, Ruslan
AU - Nudler, Evgeny
PY - 2002/4/30
Y1 - 2002/4/30
N2 - Nitric oxide (NO') is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO concentrations, plasma albumin becomes saturated with NO and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO and its reactive oxides and controls the dynamics of NO -dependant processes in the vasculature.
AB - Nitric oxide (NO') is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO concentrations, plasma albumin becomes saturated with NO and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO and its reactive oxides and controls the dynamics of NO -dependant processes in the vasculature.
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U2 - 10.1073/pnas.092048999
DO - 10.1073/pnas.092048999
M3 - Article
C2 - 11983891
AN - SCOPUS:0037197840
SN - 0027-8424
VL - 99
SP - 5913
EP - 5918
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -