Catalysis of S-nitrosothiols formation by serum albumin: The mechanism and implication in vascular control

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Abstract

Nitric oxide (NO') is a short-lived physiological messenger. Its various biological activities can be preserved in a more stable form of S-nitrosothiols (RS-NO). Here we demonstrate that at physiological NO concentrations, plasma albumin becomes saturated with NO and accelerates formation of low-molecular-weight (LMW) RS-NO in vitro and in vivo. The mechanism involves micellar catalysis of NO oxidation in the albumin hydrophobic core and specific transfer of NO+ to LMW thiols. Albumin-mediated S-nitrosylation and its vasodilatory effect directly depend on the concentration of circulating LMW thiols. Results suggest that the hydrophobic phase formed by albumin serves as a major reservoir of NO and its reactive oxides and controls the dynamics of NO -dependant processes in the vasculature.

Original languageEnglish (US)
Pages (from-to)5913-5918
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number9
DOIs
StatePublished - Apr 30 2002
Externally publishedYes

ASJC Scopus subject areas

  • General

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