Ca2+-ATPase activity in the human Lens

Douglas Borchman; Christopher A. Patenon; Nicholas A. Delamere

doi:10.3109/02713688908997397

Ca²⁺-ATPase activity in the human Lens

Douglas Borchman
, Christopher A. Patenon
, Nicholas A. Delamere

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10^-6 M. Ca²⁺-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca²⁺-ATPase activity was observed with increasing age concomitant with a general increase in Ca²⁺-ATPase activity suggesting age related modifications of the membrane.

Original language	English (US)
Pages (from-to)	1049-1054
Number of pages	6
Journal	Current Eye Research
Volume	8
Issue number	10
DOIs	https://doi.org/10.3109/02713688908997397
State	Published - 1989
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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@article{2ff17a1539854e8da32ba717cc16be83,

title = "Ca2+-ATPase activity in the human Lens",

abstract = "A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85\% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.",

author = "Douglas Borchman and Patenon, \{Christopher A.\} and Delamere, \{Nicholas A.\}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by USPHS research grants EY06916 and EY06917 from the National Eye Institute, NIH, the Kentucky Lions Eye Foundation and an unrestricted grant from Research to Prevent Blindness, Inc.",

year = "1989",

doi = "10.3109/02713688908997397",

language = "English (US)",

volume = "8",

pages = "1049--1054",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Taylor and Francis Ltd.",

number = "10",

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TY - JOUR

T1 - Ca2+-ATPase activity in the human Lens

AU - Borchman, Douglas

AU - Patenon, Christopher A.

AU - Delamere, Nicholas A.

N1 - Funding Information: ACKNOWLEDGEMENTS This work was supported by USPHS research grants EY06916 and EY06917 from the National Eye Institute, NIH, the Kentucky Lions Eye Foundation and an unrestricted grant from Research to Prevent Blindness, Inc.

PY - 1989

Y1 - 1989

N2 - A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.

AB - A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.

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DO - 10.3109/02713688908997397

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AN - SCOPUS:0024471693

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SP - 1049

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JO - Current Eye Research

JF - Current Eye Research

IS - 10

ER -

Ca²⁺-ATPase activity in the human Lens

Abstract

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