TY - JOUR
T1 - Ca2+-ATPase activity in the human Lens
AU - Borchman, Douglas
AU - Patenon, Christopher A.
AU - Delamere, Nicholas A.
N1 - Funding Information:
ACKNOWLEDGEMENTS This work was supported by USPHS research grants EY06916 and EY06917 from the National Eye Institute, NIH, the Kentucky Lions Eye Foundation and an unrestricted grant from Research to Prevent Blindness, Inc.
PY - 1989
Y1 - 1989
N2 - A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.
AB - A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.
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U2 - 10.3109/02713688908997397
DO - 10.3109/02713688908997397
M3 - Article
C2 - 2533056
AN - SCOPUS:0024471693
SN - 0271-3683
VL - 8
SP - 1049
EP - 1054
JO - Current Eye Research
JF - Current Eye Research
IS - 10
ER -