Ca2+-ATPase activity in the human Lens

Douglas Borchman, Christopher A. Patenon, Nicholas A. Delamere

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.

Original languageEnglish (US)
Pages (from-to)1049-1054
Number of pages6
JournalCurrent Eye Research
Issue number10
StatePublished - 1989

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Ca2+-ATPase activity in the human Lens'. Together they form a unique fingerprint.

Cite this