Abstract
Mouse genetic engineering has revolutionized our understanding of the molecular and genetic basis of heart development and disease. This technology involves conditional tissue-specific and temporal transgenic and gene targeting approaches, as well as introduction of polymorphisms into the mouse genome. These approaches are increasingly used to elucidate the genetic pathways underlying tissue homeostasis, physiology, and pathophysiology of adult heart. They have also led to the development of clinically relevant models of human cardiac diseases. Here, we review the technologies and their limitations in general and the cardiovascular research community in particular.
Original language | English (US) |
---|---|
Pages (from-to) | 1498-1512 |
Number of pages | 15 |
Journal | Circulation research |
Volume | 110 |
Issue number | 11 |
DOIs | |
State | Published - May 25 2012 |
Externally published | Yes |
Keywords
- Cre recombinase
- conditional knockout mice
- heart
- inducible gene targeting
- transgenic mice
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine