Calcium transport by basolateral membranes of diabetic rats

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6 Scopus citations


The current study was designed to examine calcium transport across the basolateral membrane of the enterocyte of control and diabetic rats. The slope of initial rate of ATP-dependent calcium uptake was significantly greater in controls than in diabetic rats (P < 0.001). Kinetic analysis of ATP-dependent calcium uptake showed a maximum velocity (V(max)) of 0.19 ± 0.02 and 0.37 ± 0.01 μmol · g protein-1 · s-1 in diabetic and control rats, respectively (P < 0.01). K(m) values were similar. Insulin therapy and calmodulin added to freeze-thawed basolateral membranes vesicles stimulated V(max) of ATP-dependent calcium uptake in diabetic membranes to values close to those of controls without a change in K(m) values. In contrast, the Ca++-Na+ exchange process was similar in both control and diabetic rats. These results suggest that the ATP-dependent calcium uptake process is decreased in diabetic animals. This decrease is stimulated by calmodulin or insulin therapy, implying a causal relationship between insulin deficiency and calmodulin activity in diabetes.

Original languageEnglish (US)
Pages (from-to)209-214
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Issue number2
StatePublished - 1993


  • Calcium
  • calmodulin
  • diabetes
  • insulin
  • intestine

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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