Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic

Dmitry Poteryaev, Hanna Fares, Bruce Bowerman, Anne Spang

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


The small rab-GTPase RAB-7 acts in endosome and endosome to lysosome traffic. We identified SAND-1 as a protein required for RAB-7 function based on similarities between SAND-1 and RAB-7 RNAi phenotypes. Although the initial uptake of yolk protein in oocytes, or of soluble secreted (ss) GFP in coelomocytes, appeared normal, further transport along the endocytic traffic route was delayed in the absence of SAND-1 function, and yolk proteins failed to reach yolk granules efficiently. Moreover, in coelomocytes, ssGFP and BSA-Texas-Red were endocytosed but not transported to lysosomes. We show that SAND-1 is essential for RAB-7 function at the transition from early to late endosomes, but not for RAB-7 function at lysosomes.

Original languageEnglish (US)
Pages (from-to)301-312
Number of pages12
JournalEMBO Journal
Issue number2
StatePublished - Jan 24 2007
Externally publishedYes


  • C. elegans
  • Early to late endosome transport
  • Endosomes
  • RAB GTPases
  • RAB7

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


Dive into the research topics of 'Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic'. Together they form a unique fingerprint.

Cite this