Caenorhabditis elegans functional orthologue of human protein h-mucolipin-1 is required for lysosome biogenesis

Sebastian Treusch, Sarah Knuth, Susan A. Slaugenhaupt, Ehud Goldin, Barth D. Grant, Hanna Fares

Research output: Contribution to journalArticlepeer-review

189 Scopus citations


Mucolipidosis type IV (MLIV) is an autosomal recessive lysosomal storage disease characterized by severe psychomotor retardation, achlorhydria, and ophthalmological abnormalities. Cells from several tissues in MLIV patients accumulate large vacuoles that are presumed to be lysosomes, but whose exact nature remains to be determined. Other defects include the deterioration of neuronal integrity in the retina and the cerebellum. MCOLN1, the gene mutated in MLIV patients, encodes a protein called h-mucolipin-1 that has six predicted transmembrane domains and functions as a Ca2+-permeable channel that is modulated by changes in Ca2+ concentration. CUP-5 is the Caenorhabditis elegans functional orthologue of h-mucolipin-1. Mutations in cup-5 result in the accumulation of large vacuoles in several cells, in increased cell death, and in embryonic lethality. We demonstrate here that CUP-5 functions in the biogenesis of lysosomes originating from hybrid organelles. We also show that at least two h-mucolipin family members rescue cup-5 mutant endocytic defects, indicating that there may be functional redundancy among the human proteins. Finally, we propose a model that relates the lysosome biogenesis defect in the absence of CUP-5/h-mucolipin-1 to cellular phenotypes in worms and in humans.

Original languageEnglish (US)
Pages (from-to)4483-4488
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number13
StatePublished - Mar 30 2004
Externally publishedYes


  • CUP-5
  • Mucolipidosis type IV

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Caenorhabditis elegans functional orthologue of human protein h-mucolipin-1 is required for lysosome biogenesis'. Together they form a unique fingerprint.

Cite this