Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

Sergio B. Mendes; Lifeng Li; James J. Burke; John E. Lee; Darren R. Dunphy; S. Scott Saavedra

doi:10.1021/la9601162

Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

Sergio B. Mendes, Lifeng Li, James J. Burke, John E. Lee, Darren R. Dunphy, S. Scott Saavedra

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular film at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nm bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.

Original language	English (US)
Pages (from-to)	3374-3376
Number of pages	3
Journal	Langmuir
Volume	12
Issue number	14
DOIs	https://doi.org/10.1021/la9601162
State	Published - Jul 10 1996
Externally published	Yes

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la9601162

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abstract = "The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular film at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nm bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.",

author = "Mendes, {Sergio B.} and Lifeng Li and Burke, {James J.} and Lee, {John E.} and Dunphy, {Darren R.} and Saavedra, {S. Scott}",

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T1 - Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

AU - Mendes, Sergio B.

AU - Li, Lifeng

AU - Burke, James J.

AU - Lee, John E.

AU - Dunphy, Darren R.

AU - Saavedra, S. Scott

PY - 1996/7/10

Y1 - 1996/7/10

N2 - The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular film at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nm bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.

AB - The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular film at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nm bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.

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Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

Abstract

ASJC Scopus subject areas

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