Abstract
We have constructed an expression library of Bordetella pertussis DNA sequences, cloned in Escherichia coli. Random DNA fragments were inserted into the beta-galactosidase gene of bacteriophage lambda gt11 and plaques were screened with antibodies directed against filamentous hemagglutinin. The antigen-positive clone obtained expressed B. pertussis sequences as polypeptides fused to beta-galactosidase. The fusion polypeptides reacted both with antibodies to beta-galactosidase and with antibodies to filamentous hemagglutinin. The positive clone contains B. pertussis DNA sequences as determined by specific hybridization to Bordetella DNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 265-271 |
| Number of pages | 7 |
| Journal | Developments in biological standardization |
| Volume | 61 |
| State | Published - 1985 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Immunology and Microbiology
- Drug Discovery
- Public Health, Environmental and Occupational Health