Abstract
We have constructed an expression library of Bordetella pertussis DNA sequences, cloned in Escherichia coli. Random DNA fragments were inserted into the beta-galactosidase gene of bacteriophage lambda gt11 and plaques were screened with antibodies directed against filamentous hemagglutinin. The antigen-positive clone obtained expressed B. pertussis sequences as polypeptides fused to beta-galactosidase. The fusion polypeptides reacted both with antibodies to beta-galactosidase and with antibodies to filamentous hemagglutinin. The positive clone contains B. pertussis DNA sequences as determined by specific hybridization to Bordetella DNA.
Original language | English (US) |
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Pages (from-to) | 265-271 |
Number of pages | 7 |
Journal | Developments in biological standardization |
Volume | 61 |
State | Published - 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- General Immunology and Microbiology
- Drug Discovery
- Public Health, Environmental and Occupational Health