TY - JOUR
T1 - Bordetella pertussis adenylate cyclase
T2 - Effects of affinity-purified adenylate cyclase on human polymorphonuclear leukocyte functions
AU - Friedman, R. L.
AU - Fiederlein, R. L.
AU - Glasser, L.
AU - Galgiani, J. N.
PY - 1987
Y1 - 1987
N2 - Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrted to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 μg/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50% inhibitory dose for chemotaxis and chemiluminescence was 36.5 μg/ml; for superoxide generation it was 71.0 μg/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.
AB - Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrted to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 μg/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50% inhibitory dose for chemotaxis and chemiluminescence was 36.5 μg/ml; for superoxide generation it was 71.0 μg/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.
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U2 - 10.1128/iai.55.1.135-140.1987
DO - 10.1128/iai.55.1.135-140.1987
M3 - Article
C2 - 2878884
AN - SCOPUS:0023088297
SN - 0019-9567
VL - 55
SP - 135
EP - 140
JO - Infection and Immunity
JF - Infection and Immunity
IS - 1
ER -