Biophysics of Membrane Stiffening by Cholesterol and Phosphatidylinositol 4,5-bisphosphate (PIP2)

Fathima T. Doole, Sudipta Gupta, Teshani Kumarage, Rana Ashkar, Michael F. Brown

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Cell membranes regulate a wide range of phenomena that are implicated in key cellular functions. Cholesterol, a critical component of eukaryotic cell membranes, is responsible for cellular organization, membrane elasticity, and other critical physicochemical parameters. Besides cholesterol, other lipid components such as phosphatidylinositol 4,5-bisphosphate (PIP2) are found in minor concentrations in cell membranes yet can also play a major regulatory role in various cell functions. In this chapter, we describe how solid-state deuterium nuclear magnetic resonance (2H NMR) spectroscopy together with neutron spin-echo (NSE) spectroscopy can inform synergetic changes to lipid molecular packing due to cholesterol and PIP2 that modulate the bending rigidity of lipid membranes. Fundamental structure–property relations of molecular self-assembly are illuminated and point toward a length and time-scale dependence of cell membrane mechanics, with significant implications for biological activity and membrane lipid–protein interactions.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer
Pages61-85
Number of pages25
DOIs
StatePublished - 2023

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1422
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Area per lipid
  • Cholesterol
  • Membrane elasticity
  • Membrane viscosity
  • Neutron spin-echo
  • PIP2
  • Polymer brush model
  • Solid-stateH NMR

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

Cite this