Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas)

Shane A. Snyder, Timothy L. Keith, Susan L. Pierens, Erin M. Snyder, John P. Giesy

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μg NP/1 in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reversephase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.

Original languageEnglish (US)
Pages (from-to)1697-1702
Number of pages6
Issue number8
StatePublished - 2001


  • Alkylphenol
  • Bioaccumulation
  • Bioconcentration factor
  • Surfactant

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis


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