Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas)

Shane A. Snyder; Timothy L. Keith; Susan L. Pierens; Erin M. Snyder; John P. Giesy

doi:10.1016/S0045-6535(00)00524-5

Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas)

Shane A. Snyder, Timothy L. Keith, Susan L. Pierens, Erin M. Snyder, John P. Giesy

Chemical and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μg NP/1 in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reversephase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.

Original language	English (US)
Pages (from-to)	1697-1702
Number of pages	6
Journal	Chemosphere
Volume	44
Issue number	8
DOIs	https://doi.org/10.1016/S0045-6535(00)00524-5
State	Published - 2001

Keywords

Alkylphenol
Bioaccumulation
Bioconcentration factor
Surfactant

ASJC Scopus subject areas

Environmental Engineering
Environmental Chemistry
General Chemistry
Pollution
Health, Toxicology and Mutagenesis

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10.1016/S0045-6535(00)00524-5

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title = "Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas)",

abstract = "Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μg NP/1 in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reversephase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.",

keywords = "Alkylphenol, Bioaccumulation, Bioconcentration factor, Surfactant",

author = "Snyder, {Shane A.} and Keith, {Timothy L.} and Pierens, {Susan L.} and Snyder, {Erin M.} and Giesy, {John P.}",

note = "Funding Information: This work was supported by the Chlorine Chemical Council of the Chemical Manufacturer's Association, the National Institute of Environmental Health Sciences (NIEHS) Superfund Basic Research Program (ES0491), and the U.S. Environmental Protection Agency (EPA) Office of Water Quality (CR 822983-01-0). Shane Snyder received his Ph.D. from Michigan State University's Department of Zoology and Institute for Environmental Toxicology. His research focus has been the development of instrumental and bioanalytical measures of endocrine disruptors in water. Most recently, he has conducted extensive research on bioactive compounds present in the waters of Lake Mead, Nevada. ",

year = "2001",

doi = "10.1016/S0045-6535(00)00524-5",

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AU - Snyder, Shane A.

AU - Keith, Timothy L.

AU - Pierens, Susan L.

AU - Snyder, Erin M.

AU - Giesy, John P.

N1 - Funding Information: This work was supported by the Chlorine Chemical Council of the Chemical Manufacturer's Association, the National Institute of Environmental Health Sciences (NIEHS) Superfund Basic Research Program (ES0491), and the U.S. Environmental Protection Agency (EPA) Office of Water Quality (CR 822983-01-0). Shane Snyder received his Ph.D. from Michigan State University's Department of Zoology and Institute for Environmental Toxicology. His research focus has been the development of instrumental and bioanalytical measures of endocrine disruptors in water. Most recently, he has conducted extensive research on bioactive compounds present in the waters of Lake Mead, Nevada.

PY - 2001

Y1 - 2001

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AB - Bioconcentration of p-nonylphenol (NP) by fathead minnows was determined under laboratory conditions. Fish were exposed continuously for 42 days to 0.33, 0.93 and 2.36 μg NP/1 in a flow-through system. NP was Soxhlet extracted from whole fish homogenates with dichloromethane (DCM). The resulting extract was concentrated and bulk lipids removed by gel permeation and silica-gel chromatography. Compounds were identified and quantified by reversephase high-pressure liquid chromatography (RP-HPLC) with fluorescence detection. Mass spectrometry was used for verification of peak assignments. Bioconcentration factors (BCFs) ranged from 245 to 380.

KW - Alkylphenol

KW - Bioaccumulation

KW - Bioconcentration factor

KW - Surfactant

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Bioconcentration of nonylphenol in fathead minnows (Pimephales promelas)

Abstract

Keywords

ASJC Scopus subject areas

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