Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

Noah W. Palm; Rachel K. Rosenstein; Shuang Yu; Dominik D. Schenten; Esther Florsheim; Ruslan Medzhitov

doi:10.1016/j.immuni.2013.10.006

Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

Noah W. Palm, Rachel K. Rosenstein, Shuang Yu, Dominik D. Schenten, Esther Florsheim, Ruslan Medzhitov

Immunobiology

Research output: Contribution to journal › Article › peer-review

151 Scopus citations

Abstract

Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved component of venoms from multiple species and is the major allergen in bee venom. Here we examined how bee venom PLA2 is sensed by the innate immune system and induces a type 2 immune response in mice. We found that bee venom PLA2 induced a T helper type 2 (Th2) cell-type response and group 2 innate lymphoid cell activation via the enzymatic cleavage of membrane phospholipids and release of interleukin-33. Furthermore, we showed that the IgE response to PLA2 could protect mice from future challenge with a near-lethal dose of PLA2. These data suggest that the innate immune system can detect the activity of a conserved component of venoms and induce a protective immune response against a venom toxin.

Original language	English (US)
Pages (from-to)	976-985
Number of pages	10
Journal	Immunity
Volume	39
Issue number	5
DOIs	https://doi.org/10.1016/j.immuni.2013.10.006
State	Published - Nov 14 2013

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Infectious Diseases

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10.1016/j.immuni.2013.10.006

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title = "Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity",

abstract = "Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved component of venoms from multiple species and is the major allergen in bee venom. Here we examined how bee venom PLA2 is sensed by the innate immune system and induces a type 2 immune response in mice. We found that bee venom PLA2 induced a T helper type 2 (Th2) cell-type response and group 2 innate lymphoid cell activation via the enzymatic cleavage of membrane phospholipids and release of interleukin-33. Furthermore, we showed that the IgE response to PLA2 could protect mice from future challenge with a near-lethal dose of PLA2. These data suggest that the innate immune system can detect the activity of a conserved component of venoms and induce a protective immune response against a venom toxin.",

author = "Palm, {Noah W.} and Rosenstein, {Rachel K.} and Shuang Yu and Schenten, {Dominik D.} and Esther Florsheim and Ruslan Medzhitov",

note = "Funding Information: We would like to thank members of the Medzhitov laboratory for helpful discussions, C. Annicelli and S. Cronin for animal care and technical help, O. Colegio for helpful comments on the manuscript, and Andrew McKenzie, Mark Shlomchik, Janusz Kabarowski, and Vishva Dixit for making available the St2 −/− , MyD88 −/− ;BALB/c, Gpr132 −/− , Ripk3 −/− mice, respectively. This study was supported by grants from the National Institutes of Health (RO1 AI08977, 5RO1 AI055502, and R37 AI046688) and the Howard Hughes Medical Institute. ",

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AU - Schenten, Dominik D.

AU - Florsheim, Esther

AU - Medzhitov, Ruslan

N1 - Funding Information: We would like to thank members of the Medzhitov laboratory for helpful discussions, C. Annicelli and S. Cronin for animal care and technical help, O. Colegio for helpful comments on the manuscript, and Andrew McKenzie, Mark Shlomchik, Janusz Kabarowski, and Vishva Dixit for making available the St2 −/− , MyD88 −/− ;BALB/c, Gpr132 −/− , Ripk3 −/− mice, respectively. This study was supported by grants from the National Institutes of Health (RO1 AI08977, 5RO1 AI055502, and R37 AI046688) and the Howard Hughes Medical Institute.

PY - 2013/11/14

Y1 - 2013/11/14

N2 - Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved component of venoms from multiple species and is the major allergen in bee venom. Here we examined how bee venom PLA2 is sensed by the innate immune system and induces a type 2 immune response in mice. We found that bee venom PLA2 induced a T helper type 2 (Th2) cell-type response and group 2 innate lymphoid cell activation via the enzymatic cleavage of membrane phospholipids and release of interleukin-33. Furthermore, we showed that the IgE response to PLA2 could protect mice from future challenge with a near-lethal dose of PLA2. These data suggest that the innate immune system can detect the activity of a conserved component of venoms and induce a protective immune response against a venom toxin.

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Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

Abstract

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