Bcl-2 transduction protects human endothelial cell synthetic microvessell grafts from allogeneic T cells in vivo

Lian Zheng, Thomas F. Gibson, Jeffrey S. Schechner, Jordan S. Pober, Alfred L.M. Bothwell

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

T cell interactions with vascular endothelial cells (EQ are of central importance for immune surveillance of microbes and for pathological processes such as atherosclerosis, allograft rejection, and vasculitis. Animal (especially rodent) models incompletely predict human immune responses, in particular with regard to the immunological functions of EC, and in vitro models may not accurately reflect in vivo findings. In this study, we describe the development of an immunodeficient SCID/bg murine model combining a transplanted human synthetic microvascular bed with adoptive transfer of human T lymphocytes allogeneic to the cells of the graft that more fully recapitulates T cell responses in natural tissues. Using this model, we demonstrate that transduced Bcl-2 protein in the engrafted EC] effectively prevents injury even as it enhances T cell graft infiltration and replication.

Original languageEnglish (US)
Pages (from-to)3020-3026
Number of pages7
JournalJournal of Immunology
Volume173
Issue number5
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Bcl-2 transduction protects human endothelial cell synthetic microvessell grafts from allogeneic T cells in vivo'. Together they form a unique fingerprint.

Cite this