Abstract
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q m = 29.15 mg PCI/mL of adsorbent and K d = 0.0145 mg de pCI/mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k 1 = 0.0067 mL/mg s.
Original language | English (US) |
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Pages (from-to) | 196-200 |
Number of pages | 5 |
Journal | Journal of Liquid Chromatography and Related Technologies |
Volume | 38 |
Issue number | 2 |
DOIs | |
State | Published - Jan 20 2015 |
Keywords
- DNA vaccines
- adsorption isotherms
- adsorption models
- batch adsorption kinetics
- perfusion adsorption
- plasmid purification
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Science
- Clinical Biochemistry