TY - JOUR
T1 - Bacteriuria screening by direct bioluminescence assay of ATP
AU - Schifman, R. B.
AU - Wieden, M.
AU - Brooker, J.
AU - Chery, M.
AU - Delduca, M.
AU - Norgard, K.
AU - Palen, C.
AU - Reis, N.
AU - Swanson, J.
AU - White, J.
PY - 1984
Y1 - 1984
N2 - A direct bioluminescence assay for bacteriuria screening is described and compared with the MS-2 system (Abbott Laboratories, Irvine, Tex.) and the chemical strip, Gram smear, and calibrated-loop methods. A total of 973 specimens were tested. Unlike previously described bioluminescence methods, this test measures total ATP in urine without pretreatment of samples to remove somatic ATP. The result was compared with an ATP standard (20 ng/ml). A low result (<3% of standard) was interpreted as negative and a high result (>10% of standard) as positive. Samples with intermediate results (38% of total) were incubated at 35°C in thioglycolate broth (1:10). A 2% increase in ATP concentration was interpreted as positive. The sensitivity of this method for detecting >105 pathogens per ml was 92.3% and was comparable to those of the MS-2 system (92.7%) and the Gram smear method (90.5%). The chemical strip method was less sensitive (84.0%). The direct bioluminescence method was more sensitive than were the MS-2 system and the Gram smear method for detecting low-level bacteriuria (<103 to 105 organisms per ml), primarily because of associated pyuria. Thioglycolate broth provided a suitable medium for ATP production, and 5% CO2 decreased bacterial ATP synthesis during log-phase growth. The direct bioluminescence assay is rapid, simple, cost-effective, and reliable for bacteriuria screening.
AB - A direct bioluminescence assay for bacteriuria screening is described and compared with the MS-2 system (Abbott Laboratories, Irvine, Tex.) and the chemical strip, Gram smear, and calibrated-loop methods. A total of 973 specimens were tested. Unlike previously described bioluminescence methods, this test measures total ATP in urine without pretreatment of samples to remove somatic ATP. The result was compared with an ATP standard (20 ng/ml). A low result (<3% of standard) was interpreted as negative and a high result (>10% of standard) as positive. Samples with intermediate results (38% of total) were incubated at 35°C in thioglycolate broth (1:10). A 2% increase in ATP concentration was interpreted as positive. The sensitivity of this method for detecting >105 pathogens per ml was 92.3% and was comparable to those of the MS-2 system (92.7%) and the Gram smear method (90.5%). The chemical strip method was less sensitive (84.0%). The direct bioluminescence method was more sensitive than were the MS-2 system and the Gram smear method for detecting low-level bacteriuria (<103 to 105 organisms per ml), primarily because of associated pyuria. Thioglycolate broth provided a suitable medium for ATP production, and 5% CO2 decreased bacterial ATP synthesis during log-phase growth. The direct bioluminescence assay is rapid, simple, cost-effective, and reliable for bacteriuria screening.
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U2 - 10.1128/jcm.20.4.644-648.1984
DO - 10.1128/jcm.20.4.644-648.1984
M3 - Article
C2 - 6490851
AN - SCOPUS:0021182660
SN - 0095-1137
VL - 20
SP - 644
EP - 648
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 4
ER -