Bacterial diversity of diabetic foot ulcers: Current status and future prospectives

Fatemah Sadeghpour Heravi, Martha Zakrzewski, Karen Vickery, David G. Armstrong, Honghua Hu

Research output: Contribution to journalReview articlepeer-review

65 Scopus citations

Abstract

Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosiS. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganismS. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA-and RNA-based techniques for bacterial identification can improve therapeutic approacheS. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches.

Original languageEnglish (US)
Article number1935
JournalJournal of Clinical Medicine
Volume8
Issue number11
DOIs
StatePublished - Nov 2019

Keywords

  • 16S rRNA sequencing
  • Culture
  • Culturomics
  • Diabetic foot infections
  • Diabetic foot ulcers
  • Metagenomics
  • Metatranscriptomics
  • Microbiology
  • Microbiota

ASJC Scopus subject areas

  • General Medicine

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