Abstract
Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosiS. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganismS. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA-and RNA-based techniques for bacterial identification can improve therapeutic approacheS. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches.
Original language | English (US) |
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Article number | 1935 |
Journal | Journal of Clinical Medicine |
Volume | 8 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2019 |
Keywords
- 16S rRNA sequencing
- Culture
- Culturomics
- Diabetic foot infections
- Diabetic foot ulcers
- Metagenomics
- Metatranscriptomics
- Microbiology
- Microbiota
ASJC Scopus subject areas
- General Medicine