@article{4bcaa8863d7d41e2bcb0b715a5aed447,
title = "Automatic reagent handling and assay processing of human biospecimens inside a transportation container for a medical disaster response against radiation",
abstract = "Biological materials can be shipped off-site for diagnostic, therapeutic and research purposes. They usually are kept in certain environments for their final application during transportation. However, active reagent handling during transportation from a collection site to a laboratory or biorepository has not been reported yet. In this paper, we show the application of a micro-controlled centrifugal microfluidic system inside a shipping container that can add reagent to an actively cultured human blood sample during transportation to ensure a rapid biodosimetry of cytokinesis-block micronucleus (CBMN) assay. The newly demonstrated concept could have a significant impact on rapid biodosimetry triage for medical countermeasure in a radiological disaster. It also opens a new capability in accelerated sample processing during transportation for biomedical and healthcare applications.",
author = "Akkad, {Adam R.} and Jian Gu and Brett Duane and Alan Norquist and Brenner, {David J.} and Adarsh Ramakumar and Frederic Zenhausern",
note = "Funding Information: The funding was awarded to J.G. and F.Z. by a subaward (Agreement No.: 3042) from Armed Forces Radiobiology Research Institute through a Congressionally Directed Medical Research Program, Merit Based Discovery Award to A.R. (Award# W81XWH-15-2-0076, Grant PR142006). The funders (other than the named authors) had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors would like to thank Baiju Thomas at The University of Arizona for helping with plastic part fabrication, Jamie Cox at HonorHealth Research and Innovation Institute for receiving and shipping the SSI, and the NIH/NIAID Center for Medical Countermeasures Against Radiation (CMCR) program (Project Number 5U19AI067773) for CBMN assay protocol. Publisher Copyright: Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.",
year = "2022",
month = may,
doi = "10.1371/journal.pone.0268508",
language = "English (US)",
volume = "17",
journal = "PloS one",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5 May",
}