Automated microscope-independent fluorescence-guided micropipette

Christopher Miranda, Madeleine R. Howell, Joel F. Lusk, Ethan Marschall, Jarrett Eshima, Trent Anderson, Barbara S. Smith

Research output: Contribution to journalArticlepeer-review

Abstract

Glass micropipette electrodes are commonly used to provide high resolution recordings of neurons. Although it is the gold standard for single cell recordings, it is highly dependent on the skill of the electrophysiologist. Here, we demonstrate a method of guiding micropipette electrodes to neurons by collecting fluorescence at the aperture, using an intra-electrode tapered optical fiber. The use of a tapered fiber for excitation and collection of fluorescence at the micropipette tip couples the feedback mechanism directly to the distance between the target and electrode. In this study, intra-electrode tapered optical fibers provide a targeted robotic approach to labeled neurons that is independent of microscopy.

Original languageEnglish (US)
Article number#431372
JournalBiomedical Optics Express
Volume12
Issue number8
DOIs
StatePublished - Aug 1 2021
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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