Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

Weiguo Qiu; Joseph Cappello; Xiaoyi Wu

doi:10.1063/1.3604786

Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

Weiguo Qiu, Joseph Cappello, Xiaoyi Wu

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.

Original language	English (US)
Article number	263702
Journal	Applied Physics Letters
Volume	98
Issue number	26
DOIs	https://doi.org/10.1063/1.3604786
State	Published - Jun 27 2011

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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title = "Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers",

abstract = "We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.",

author = "Weiguo Qiu and Joseph Cappello and Xiaoyi Wu",

note = "Funding Information: This work was supported by the NIH (EB009801) and American Heart Association (09BGIA2250621).",

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T1 - Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

AU - Qiu, Weiguo

AU - Cappello, Joseph

AU - Wu, Xiaoyi

N1 - Funding Information: This work was supported by the NIH (EB009801) and American Heart Association (09BGIA2250621).

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Y1 - 2011/6/27

N2 - We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.

AB - We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of -turns and unordered structures to ordered -sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ±0.28 MPa, an ultimate tensile strength of 0.34 ±0.04 MPa, and a strain at failure of 29% ±3%.

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Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers

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